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Proceedings Paper

Nano-aquarium with microfluidic structures fabricated by femtosecond laser direct writing for dynamic microorganism analysis
Author(s): Y. Hanada; K. Sugioka; H. Kawano; I. Ishikawa; A. Miyawaki; K. Midorikawa
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Paper Abstract

We demonstrate the fabrication of three-dimensional (3D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis and Cryptomonas. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms.

Paper Details

Date Published: 18 February 2009
PDF: 8 pages
Proc. SPIE 7276, Photonics and Optoelectronics Meetings (POEM) 2008: Laser Technology and Applications, 72760Z (18 February 2009); doi: 10.1117/12.823397
Show Author Affiliations
Y. Hanada, RIKEN-Advanced Science Institute (Japan)
K. Sugioka, RIKEN-Advanced Science Institute (Japan)
H. Kawano, RIKEN-Brain Science Institute (Japan)
I. Ishikawa, RIKEN-Brain Science Institute (Japan)
A. Miyawaki, RIKEN-Brain Science Institute (Japan)
K. Midorikawa, RIKEN-Advaced Science Institute (Japan)

Published in SPIE Proceedings Vol. 7276:
Photonics and Optoelectronics Meetings (POEM) 2008: Laser Technology and Applications
Peixiang Lu; Katsumi Midorikawa; Bernd Wilhelmi, Editor(s)

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