Share Email Print
cover

Proceedings Paper

Microsphere-enhanced label-free high-throughput molecular detection
Author(s): Cheng Deng; Guoliang Huang; Shukuan Xu; Jing Zhu; Rui Ma; Chao Han; Shengyi Chen; Chen Ma
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In this paper, we developed a microsphere enhanced and label free high throughput molecular detection based on SPRI and microarray chips, and a self-built surface plasmon resonance imaging instrument was set. One label-free protein chip forming a 7× probe array was designed and fabricated using a commercial microarray robot spotter on chemical modified gold-coated glass slide. Antibody molecules were successfully detected in label-free and high-throughput method by using this chip. The detection signals on the chip was successfully enhanced by using microspheres with a diameter of 1 μm.

Paper Details

Date Published: 6 March 2009
PDF: 7 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800S (6 March 2009); doi: 10.1117/12.823373
Show Author Affiliations
Cheng Deng, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Guoliang Huang, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Shukuan Xu, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Jing Zhu, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Rui Ma, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Chao Han, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Shengyi Chen, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Chen Ma, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top