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Proceedings Paper

Development of a spectrally resolved multifocal multiphoton microscope
Author(s): Lixin Liu; Yonghong Shao; Junle Qu; Heng Li; Baoping Guo; Wenqing Liu; Hanben Niu
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Paper Abstract

Multifocal Multiphoton Microscopy (MMM) can acquire three-dimensional (3D) fluorescence microscopic images of samples by multiphoton excitation with the advantages of high speed, reduced photobleaching, enhanced penetration depth and high signal-to-noise ratio. As fluorescence spectrum can provide information about the components of the sample, it is becoming increasingly popular in biomedicine to combine fluorescence spectrum measurement with multidimensional fluorescence microscopy. In this paper, we present the development of a spectrally resolved multifocal multiphoton microscope (SR-MMM). A microlens array is employed in the MMM system to produce 2D excitation foci on the sample for simultaneous two-photon excitation and a pair of galvo mirrors is used to scan the excitation foci across the sample. A liquid crystal tunable filter (LCTF) is mounted in front of a high-speed cooled CCD camera and is used to change the detection wavelength of the MMM system. Depth-resolved and spectrum-resolved two-photon excitation fluorescence images of a few samples are obtained with the SR-MMM system.

Paper Details

Date Published: 6 March 2009
PDF: 7 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801C (6 March 2009); doi: 10.1117/12.823366
Show Author Affiliations
Lixin Liu, Shenzhen Univ. (China)
Anhui Institute of Optics and Fine Mechanics (China)
Yonghong Shao, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Heng Li, Shenzhen Univ. (China)
Baoping Guo, Shenzhen Univ. (China)
Wenqing Liu, Anhui Institute of Optics and Fine Mechanics (China)
Hanben Niu, Shenzhen Univ. (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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