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Proceedings Paper

In-situ analysis of fruit anthocyanins by means of total internal reflectance, continuous wave and time-resolved spectroscopy
Author(s): Manuela Zude; Lorenzo Spinelli; Carsten Dosche; Alessandro Torricelli
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Paper Abstract

In sweet cherry (Prunus avium), the red pigmentation is correlated with the fruit maturity stage and can be measured by non-invasive spectroscopy. In the present study, the influence of varying fruit scattering coefficients on the fruit remittance spectrum (cw) were corrected with the effective pathlength and refractive index in the fruit tissue obtained with distribution of time-of-flight (DTOF) readings and total internal reflection fluorescence (TIRF) analysis, respectively. The approach was validated on fruits providing variation in the scattering coefficient outside the calibration sample set. In the validation, the measuring uncertainty when non-invasively analyzing fruits with cw method in comparison with combined application of cw, DTOF, and TIRF measurements showed an increase in r2 up to 22.7 % with, however, high errors in all approaches.

Paper Details

Date Published: 10 September 2009
PDF: 8 pages
Proc. SPIE 7432, Optical Inspection and Metrology for Non-Optics Industries, 74320H (10 September 2009); doi: 10.1117/12.823157
Show Author Affiliations
Manuela Zude, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (Germany)
Lorenzo Spinelli, Politecnico di Milano (Italy)
Carsten Dosche, Univ. of Potsdam (Germany)
Alessandro Torricelli, Politecnico di Milano (Italy)


Published in SPIE Proceedings Vol. 7432:
Optical Inspection and Metrology for Non-Optics Industries
Peisen S. Huang; Toru Yoshizawa; Kevin G. Harding, Editor(s)

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