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Proceedings Paper

Vascular effects induced by anti-VEGF agents in the CAM model: effect of the DMSO
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Paper Abstract

The chicken embryo's chorioallantoic membrane (CAM) is widely used as an in vivo model to study the vascular effects induced by agents administrated topically or intravenously. Hence, in the vascular plexus of this respiratory membrane, angiogenic and anti-angiogenic agents, as well as phototoxic effects have been studied. The main goal of this study was to characterize the capillary network of the CAM after topical administration of dimethyl sulfoxid (DMSO), a frequently used solvent of lipophylic drugs, including potent anti-VEGF agents. The CAM capillaries were observed between days 8 and 9 of the embryo development, with an epi-fluorescence microscope equipped with a sensitive camera by intravenous injection of a fluorescent agent and a non-fluorescing absorber (in the extra-embryonic cavity) to screen the tissue background fluorescence. The fluorescence images of the CAM vasculature were then processed in order to obtain a skeleton of the vessels and capillaries. This was done to quantify descriptors such as the number of branching points/mm2, the mean area value of the vessels network meshes, and the mean of the 3rd quartile of the histogram of these meshes, were then extracted. Our results demonstrate that the topical administration of an aqueous solution of 20 μl of DMSO at concentrations equal or larger than 0.1% turned out to modify the capillary network morphology in a dose-dependent manner as compared to the control (20 μl of 0.9% NaCl).

Paper Details

Date Published: 13 July 2009
PDF: 5 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73803W (13 July 2009); doi: 10.1117/12.822967
Show Author Affiliations
Patrycja Nowak-Sliwinska, Swiss Federal Institute of Technology (Switzerland)
Jean-Pierre Ballini, Swiss Federal Institute of Technology (Switzerland)
Hubert van den Bergh, Swiss Federal Institute of Technology (Switzerland)
Georges Wagnières, Swiss Federal Institute of Technology (Switzerland)

Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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