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Proceedings Paper

In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region
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Paper Abstract

In this study, a ratiometric quantification method is developed and applied to monitor mesotetra(hydroxyphenyl) chlorin (mTHPC) pharmacokinetics in the rat skin-fold observation chamber. The method employs a combination of dual-wavelength excitation and dual-wavelength detection. The excitation and detection wavelengths were selected in close to NIR. The first excitation wavelength was used to excite the mTHPC and autofluorescence and the second to excite only autofluorescence, so that this could be substracted. Subsequently the difference was divided by the autofluorescence. Since the method applies division of signal with no mTHPC fluorescence, theory suggests on linear dependency of the method on photosensitizer concentration.

Paper Details

Date Published: 14 July 2009
PDF: 8 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738063 (14 July 2009); doi: 10.1117/12.822947
Show Author Affiliations
Slávka Kascáková, Erasmus Medical Ctr. (Netherlands)
Sebastiaan de Visscher, Univ. Medical Ctr. Groningen (Netherlands)
Bastiaan Kruijt, Erasmus Medical Ctr. (Netherlands)
Henriëtte S. de Bruijn, Erasmus Medical Ctr. (Netherlands)
Angélique van der Ploeg-van den Heuvel, Erasmus Medical Ctr. (Netherlands)
Henricus J. C. M. Sterenborg, Erasmus Medical Ctr. (Netherlands)
Max J. H. Witjes, Univ. Medical Ctr. Groningen (Netherlands)
Arjen Amelink, Erasmus Medical Ctr. (Netherlands)
Univ. Medical Ctr. Groningen (Netherlands)
Dominic J. Robinson, Erasmus Medical Ctr. (Netherlands)

Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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