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Proceedings Paper

Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements
Author(s): Steffen Hackbarth; Jan Schlothauer; Annegret Preuß; Beate Röder
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Paper Abstract

The consumption of singlet oxygen quenchers during illumination of cells incubated with photosensitizers is a serious issue for the determination of singlet oxygen kinetics in cells. Using LNCaP cells incubated with pheophorbide a it will be shown, that already an illumination of 100 nJ/cell dramatically changes the value of the observables. This finding has consequences for the spatial resolution of any time-resolved singlet oxygen measurement, since the radiative rate constant of singlet oxygen in aqueous solutions is very small.

Paper Details

Date Published: 13 July 2009
PDF: 7 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738045 (13 July 2009); doi: 10.1117/12.822830
Show Author Affiliations
Steffen Hackbarth, Humboldt-Univ. zu Berlin (Germany)
Jan Schlothauer, Humboldt-Univ. zu Berlin (Germany)
Annegret Preuß, Humboldt-Univ. zu Berlin (Germany)
Beate Röder, Humboldt-Univ. zu Berlin (Germany)

Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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