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Proceedings Paper

Three-dimensional image formation under single-photon ultra-short pulsed illumination
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Paper Abstract

The major thrust of modern day fluorescence laser-scanning microscopy have been towards achieving better and better depth resolution embodied by the invention and subsequent development of confocal and multi-photon microscopic techniques. However, each method bears its own limitations: in having sufficient background fluorescence and photodamage resulting from out-of-focus illumination for the former, while low multi-photon absorption cross-sections of common fluorophores for the latter. Here we show how the intelligent choice of single-photon ultrashort pulsed illumination can circumvent all these shortcomings by exemplifying the tiny spatial stretch of an ultrashort pulse. Besides achieving a novel way of optical sectioning, this new method offers improved signal-to-noise ratio as well as reduced photo-damage which are crucial for live cell imaging under prolonged exposure to light.

Paper Details

Date Published: 22 May 2009
PDF: 4 pages
Proc. SPIE 7378, Scanning Microscopy 2009, 737827 (22 May 2009); doi: 10.1117/12.822773
Show Author Affiliations
Arijit Kumar De, Indian Institute of Technology Kanpur (India)
Debabrata Goswami, Indian Institute of Technology Kanpur (India)


Published in SPIE Proceedings Vol. 7378:
Scanning Microscopy 2009
Michael T. Postek; Michael T. Postek; Michael T. Postek; Dale E. Newbury; Dale E. Newbury; Dale E. Newbury; S. Frank Platek; S. Frank Platek; S. Frank Platek; David C. Joy; David C. Joy; David C. Joy, Editor(s)

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