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Proceedings Paper

A low conductivity culture medium suitable for the evaluation of sperm motility
Author(s): Rui Ma; Chao Han; Zilong Sun; Guoliang Huang; Zhongyao Yu; Yuxiang Zhou; Jundong Wang; Jie Qiao; Jing Cheng
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Paper Abstract

A novel culture medium of low conductivity suitable for dielectrophoretic selection of sperm was developed. Conventional IVF methods lack the capability of selecting the expected sperms and the embryonic development may be adversely affected to certain extent. Dielectrophoresis (DEP), a technique commonly applied in cell manipulation [1], may provide an alternative. However, the conventional IVF medium has a high conductivity, which may result in the unexpected heating effect during DEP causing damage to the gametes. The newly developed medium consists of sucrose, HEPES, BSA and low concentrations of ions. The conductivity of this medium is significantly lower than the conventional IVF medium. Motility and membrane integrality of the mouse sperm were tested in the low-conductivity medium, demonstrating an acceptable percent rate of motile sperm compared to the control groups.

Paper Details

Date Published: 6 March 2009
PDF: 7 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800H (6 March 2009); doi: 10.1117/12.822761
Show Author Affiliations
Rui Ma, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Chao Han, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Zilong Sun, Shanxi Agricultural Univ. (China)
Guoliang Huang, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Zhongyao Yu, National Engineering Research Ctr. for Beijing Biochip Technology (China)
Yuxiang Zhou, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)
Jundong Wang, Shanxi Agricultural Univ. (China)
Jie Qiao, Third Hospital of Peking Univ. (China)
Jing Cheng, Tsinghua Univ. School of Medicine (China)
National Engineering Research Ctr. for Beijing Biochip Technology (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine

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