Share Email Print
cover

Proceedings Paper

Fast detection of air contaminants using immunobiological methods
Author(s): Katrin Schmitt; Carsten Bolwien; Gerd Sulz; Wolfgang Koch; Wilhelm Dunkhorst; Hubert Lödding; Katharina Schwarz; Andreas Holländer; Torsten Klockenbring; Stefan Barth; Björn Seidel; Wolfgang Hofbauer; Torsten Rennebarth; Anna Renzl
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The fast and direct identification of possibly pathogenic microorganisms in air is gaining increasing interest due to their threat for public health, e.g. in clinical environments or in clean rooms of food or pharmaceutical industries. We present a new detection method allowing the direct recognition of relevant germs or bacteria via fluorescence-labeled antibodies within less than one hour. In detail, an air-sampling unit passes particles in the relevant size range to a substrate which contains antibodies with fluorescence labels for the detection of a specific microorganism. After the removal of the excess antibodies the optical detection unit comprising reflected-light and epifluorescence microscopy can identify the microorganisms by fast image processing on a single-particle level. First measurements with the system to identify various test particles as well as interfering influences have been performed, in particular with respect to autofluorescence of dust particles. Specific antibodies for the detection of Aspergillus fumigatus spores have been established. The biological test system consists of protein A-coated polymer particles which are detected by a fluorescence-labeled IgG. Furthermore the influence of interfering particles such as dust or debris is discussed.

Paper Details

Date Published: 18 May 2009
PDF: 12 pages
Proc. SPIE 7362, Smart Sensors, Actuators, and MEMS IV, 736207 (18 May 2009); doi: 10.1117/12.822672
Show Author Affiliations
Katrin Schmitt, Fraunhofer Institute for Physical Measurement Technique (Germany)
Carsten Bolwien, Fraunhofer Institute for Physical Measurement Technique (Germany)
Gerd Sulz, Fraunhofer Institute for Physical Measurement Technique (Germany)
Wolfgang Koch, Fraunhofer Institute of Toxicology and Experimental Medicine (Germany)
Wilhelm Dunkhorst, Fraunhofer Institute of Toxicology and Experimental Medicine (Germany)
Hubert Lödding, Fraunhofer Institute of Toxicology and Experimental Medicine (Germany)
Katharina Schwarz, Fraunhofer Institute of Toxicology and Experimental Medicine (Germany)
Andreas Holländer, Fraunhofer Institute for Applied Polymer Research (Germany)
Torsten Klockenbring, Fraunhofer Institute for Molecular Biology and Applied Ecology (Germany)
Stefan Barth, Fraunhofer Institute for Molecular Biology and Applied Ecology (Germany)
Björn Seidel, Fraunhofer Institute for Molecular Biology and Applied Ecology (Germany)
Wolfgang Hofbauer, Fraunhofer Institute for Building Physics (Germany)
Torsten Rennebarth, Fraunhofer Institute for Building Physics (Germany)
Anna Renzl, Fraunhofer Institute for Building Physics (Germany)


Published in SPIE Proceedings Vol. 7362:
Smart Sensors, Actuators, and MEMS IV
Ulrich Schmid, Editor(s)

© SPIE. Terms of Use
Back to Top