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Proceedings Paper

Comparison of ATP level in U937 cells in vitro using two different photosensitizers in photodynamic therapy
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Paper Abstract

The effect of PDT on ATP level in the Leukemic monocyte lymphoma cell line (U937) was studied using two different photosensitizers, PpIX and Hypericin. U-937 cells were irradiated (with and without ALA) and stored at room temperature. An LED lamp with a maximum peak of 637nm was used for ALA-PDT as a light source. A Na-Li lamp with a wavelength region of 560-730nm was used for Hypericin-PDT as a light source. We measured cell viability, the number of viable cells and ATP luminescence. As a result, it is evident that the irradiation time in the most effective PDT is equal to the time of the highest ATP level in the cells in ALA-PDT. This study suggests that it is possible to obtain suitable PDT conditions inducing apoptosis by measuring the ATP level in ALA-PDT. On the other hand, ATP level in Hypericin-PDT was increased with the decreasing in the cell viability. Hypericin-PDT induced apoptosis just after irradiation.

Paper Details

Date Published: 13 July 2009
PDF: 10 pages
Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73804G (13 July 2009); doi: 10.1117/12.822663
Show Author Affiliations
Nobue Nakajima, Toin Univ. of Yokohama (Japan)
Norimichi Kawashima, Toin Univ. of Yokohama (Japan)


Published in SPIE Proceedings Vol. 7380:
Photodynamic Therapy: Back to the Future
David H. Kessel, Editor(s)

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