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Proceedings Paper

Comparison of Hough and Fourier transform approach in the study of kinetics of red blood cell aggregates
Author(s): Adam Kempczyński; Maciej Bosek; Bronislaw Grzegorzewski
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Paper Abstract

The kinetics of three-dimensional red blood cell (RBC) aggregates during sedimentation using an optical method was studied. The blood was taken from healthy donors. Sedimentation of RBCs and glutaraldehyde hardened RBCs in autologous plasma and the sedimentation of RBCs in Dextran at haematocrits 5% was investigated. Light intensity in the image plane of the suspension of the cells was recorded. The movement of the aggregates was visualized by means of a spatiotemporal analysis of the light intensity. With the use of Hough transform and two-dimensional Fourier transform the velocity of the aggregates was estimated. The velocity depends on the sample height and changes with time. Spatiotemporal distributions of the aggregate velocity were determined. A comparison of velocity distributions obtained with the two methods is presented.

Paper Details

Date Published: 20 November 2008
PDF: 8 pages
Proc. SPIE 7141, 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 714118 (20 November 2008); doi: 10.1117/12.822389
Show Author Affiliations
Adam Kempczyński, Nicolaus Copernicus Univ. in Toruń (Poland)
Maciej Bosek, Nicolaus Copernicus Univ. in Toruń (Poland)
Bronislaw Grzegorzewski, Nicolaus Copernicus Univ. in Toruń (Poland)


Published in SPIE Proceedings Vol. 7141:
16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Agnieszka Popiolek-Masajada; Elzbieta Jankowska; Waclaw Urbanczyk, Editor(s)

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