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Proceedings Paper

High-throughput on-chip in vivo neural regeneration studies using femtosecond laser nano-surgery and microfluidics
Author(s): Christopher B. Rohde; Fei Zeng; Cody Gilleland; Chrysanthi Samara; Mehmet F. Yanik
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Paper Abstract

In recent years, the advantages of using small invertebrate animals as model systems for human disease have become increasingly apparent and have resulted in three Nobel Prizes in medicine or chemistry during the last six years for studies conducted on the nematode Caenorhabditis elegans (C. elegans). The availability of a wide array of species-specific genetic techniques, along with the transparency of the worm and its ability to grow in minute volumes make C. elegans an extremely powerful model organism. We present a suite of technologies for complex high-throughput whole-animal genetic and drug screens. We demonstrate a high-speed microfluidic sorter that can isolate and immobilize C. elegans in a well-defined geometry, an integrated chip containing individually addressable screening chambers for incubation and exposure of individual animals to biochemical compounds, and a device for delivery of compound libraries in standard multiwell plates to microfluidic devices. The immobilization stability obtained by these devices is comparable to that of chemical anesthesia and the immobilization process does not affect lifespan, progeny production, or other aspects of animal health. The high-stability enables the use of a variety of key optical techniques. We use this to demonstrate femtosecond-laser nanosurgery and three-dimensional multiphoton microscopy. Used alone or in various combinations these devices facilitate a variety of high-throughput assays using whole animals, including mutagenesis and RNAi and drug screens at subcellular resolution, as well as high-throughput high-precision manipulations such as femtosecond-laser nanosurgery for large-scale in vivo neural degeneration and regeneration studies.

Paper Details

Date Published: 24 February 2009
PDF: 9 pages
Proc. SPIE 7203, Commercial and Biomedical Applications of Ultrafast Lasers IX, 72030E (24 February 2009); doi: 10.1117/12.822328
Show Author Affiliations
Christopher B. Rohde, Massachusetts Institute of Technology (United States)
Fei Zeng, Massachusetts Institute of Technology (United States)
Cody Gilleland, Massachusetts Institute of Technology (United States)
Chrysanthi Samara, Massachusetts Institute of Technology (United States)
Mehmet F. Yanik, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 7203:
Commercial and Biomedical Applications of Ultrafast Lasers IX
Joseph Neev; Stefan Nolte; Alexander Heisterkamp; Rick P. Trebino, Editor(s)

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