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Proceedings Paper

Measuring dynamics of Caspase-9 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation
Author(s): Shengnan Wu; Lei Huang; Xuegang Sun; Jiru Chu
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Paper Abstract

We investigated the activity of caspase-9 for its role in the regulation of apoptosis induced by high fluence Low-power laser irradiation (HF-LPLI). Using a fluorescence resonance energy transfer (FRET) reporter STAT9, caspase-9 activity was monitored in a noninvasive technique in living human lung adenocarcinoma cells (ASTC-a-1). Under physiological conditions, proteolytic activity of caspase-9 kept invalid in order to prevent the cell undergoing apoptosis. However, HF-LPLI caused a significant decrease of Venus/ECFP ratio, indicating caspase-9 was activated which sustained from 70 minutes to 200 minutes post irradiation. This behavior was familiar with that under staurosporine (STS) treatment, which was used here as a positive control to show a characteristical activation of caspase-9. These results demonstrate that the control of caspase-9 activity is an important mechanism for the regulation of apoptosis triggered by HF-LPLI.

Paper Details

Date Published: 6 March 2009
PDF: 8 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 728023 (6 March 2009); doi: 10.1117/12.822153
Show Author Affiliations
Shengnan Wu, South China Normal Univ. (China)
Lei Huang, South China Normal Univ. (China)
Xuegang Sun, South China Normal Univ. (China)
Jiru Chu, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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