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Proceedings Paper

The role of nitric oxide in the ischemic brain evaluated by spectroscopic monitoring of mitochondrial NADH, microcirculatory blood flow, and HbO2
Author(s): Efrat Eibeshitz; Efrat Barbiro-Michaely; Avraham Mayevsky
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Paper Abstract

Nitric oxide (NO) is involved in the autoregulation of cerebral blood flow and in modulation of the respiratory chain activity in the normal brain. The present study aimed to evaluate the role of NO under two levels of partial brain ischemia induced by unilateral or bilateral carotid artery occlusion (UCO/BCO), for 24 hours and in appropriate control groups. Time-sharing fluorometer/reflectometer was used for the monitoring of mitochondrial NADH, by the fluorometric technique and oxyhemoglobin level by 2 wavelength reflectance method, combined with laser Doppler flowmetry for cerebral blood flow (CBF) monitoring. Systemic blood pressure (MAP) was also monitored. L-NAME was used for the inhibition of NO synthesis while nitrite was used as an exogenous source for NO. In both groups of ischemic animals L-NAME induced an increase in MAP and oxigenation of HbO2 however in the UCO group CBF remained stable and NADH was oxidized whereas in the BCO group CBF decreased with no change in NADH. Following nitrite infusion MAP reversibly decreased, CBF increased, HbO2 and NADH remained low in the BCO group with a slight reversible increase of NADH in the UCO group. In conclusion, it seems that NO plays an important role in the autoregulation of CBF and mitochondrial activity in the partially ischemic brain.

Paper Details

Date Published: 6 March 2009
PDF: 10 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801J (6 March 2009); doi: 10.1117/12.822132
Show Author Affiliations
Efrat Eibeshitz, Bar-Ilan Univ. (Israel)
Efrat Barbiro-Michaely, Bar-Ilan Univ. (Israel)
Avraham Mayevsky, Bar-Ilan Univ. (Israel)

Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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