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Proceedings Paper

3.0-3.7μm infrared sensor system for cell analysis
Author(s): Sander van den Driesche; Wojciech Witarski; Michael J. Vellekoop
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Paper Abstract

In this contribution we present a novel LED-photodiode based infrared absorbance sensor in the wavelength range of 3.0 - 3.7 μm for cell analysis. Instead of using time consuming and expensive labelling and staining techniques to distinguish healthy from malignant cell types, this IR sensor system can perform faster, cheaper and without the need of additional chemicals. Depending on the used narrow bandpass filters, absorbance due to specific molecular vibration can be measured, such as the functional absorbance peaks at 3.38 μm (CH3-antisymmetric stretch), 3.42 μm (CH2- antisymmetric stretch), 3.48 μm (CH3-symmetric stretch) and 3.51 μm (CH2-symmetric stretch). For normalization and baseline correction the absorbance at wavelengths 3.33 and 3.57 μm are used. By recording the IR absorbance spectra of healthy and malignant epithelial kidney cell lines with an IR spectroscope, we found significant differences in the absorbance ratio 3.51 μm / 3.42 μm (CH2-symmetric/antisymmetric stretch). This result has led us to a sensor concept where only four wavelengths are being measured. In the 3.0 - 3.7 μm wavelength region a low cost LED-photodiode system can be used instead of a spectroscope. Yeast cells, which also contain the CH2 symmetric and antisymmetric stretch bands, are used to validate this sensor system and to make a first comparison of the system to spectroscopic recordings. Sensor experiments on dried spots of baker's yeast on calcium-fluoride slides yielded a comparable CH2 stretch ratio with the IR spectroscope measurement. This confirms the usability of the sensor to measure the CH2 stretch ratio and its potential for fast, label-free and low cost screening of cell samples.

Paper Details

Date Published: 18 May 2009
PDF: 9 pages
Proc. SPIE 7362, Smart Sensors, Actuators, and MEMS IV, 73620Y (18 May 2009); doi: 10.1117/12.822058
Show Author Affiliations
Sander van den Driesche, Vienna Univ. of Technology (Austria)
Wojciech Witarski, Slovak Academy of Sciences (Slovakia)
Michael J. Vellekoop, Vienna Univ. of Technology (Austria)

Published in SPIE Proceedings Vol. 7362:
Smart Sensors, Actuators, and MEMS IV
Ulrich Schmid, Editor(s)

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