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Proceedings Paper

Sputtered polycrystalline AlN as a platform for biofunctionalized devices
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Paper Abstract

Here we present a comparison between polycrystalline AlN and (100) silicon as a support for the development of an immunosensor. A covalent approach was followed for the modification of the initially oxidized surfaces. First a layer of epoxy-based silane organic molecules was deposited. Next, protein A was immobilized with the purpose of taking advantage of its ability to properly orient the antigen binding sites of IgG antibody molecules. Finally the antibodyantigen reaction was accomplished using rabbit IgG and a corresponding antigen, such as anti-rabbit goat IgG. The antirabbit goat IgG was labelled with HRP. This allowed us to quantify the quantity of immobilized antigen. Our results demonstrate the reliability of polycrystalline AlN as a platform for immunosensing, with results comparable to those of silicon.

Paper Details

Date Published: 18 May 2009
PDF: 10 pages
Proc. SPIE 7362, Smart Sensors, Actuators, and MEMS IV, 736208 (18 May 2009); doi: 10.1117/12.821653
Show Author Affiliations
M. J. Oliver, Univ. de Castilla-La Mancha (Spain)
J. Hernando, Univ. de Castilla-La Mancha (Spain)
J. Olivares, Univ. Politécnica de Madrid (Spain)
M. Clement, Univ. Politécnica de Madrid (Spain)
A. Ababneh, Saarland Univ. (Germany)
U. Schmid, Vienna Univ. of Technology (Austria)
J. L. Sánchez-Rojas, Univ. de Castilla-La Mancha (Spain)

Published in SPIE Proceedings Vol. 7362:
Smart Sensors, Actuators, and MEMS IV
Ulrich Schmid, Editor(s)

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