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Proceedings Paper

Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR)
Author(s): F. Sonntag; S. Schmieder; N. Danz; M. Mertig; N. Schilling; U. Klotzbach; E. Beyer
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Paper Abstract

The detection of specific DNA sequences for the analysis of mutations as well as the detection of proteins gains increasing importance in the field of point-of-care diagnostics. Here, a novel low-cost lab-on-a-chip system for label-free detection of DNA hybridization and protein-protein interaction is introduced. The platform consists of a reader with disposable SPR chips produced by injection moulding. Micro optical elements are integrated into the chip to accomplish a simple connection to the optical reader. Automated, software-controlled reagent handling is achieved by a temperaturecontrolled microfluidic system comprising a syringe pump and a switching valve. The sensing area can be separated into maximum 40 parts for parallel analysis. Patterned functionalization is achieved by inverse micro contact printing. Several application examples, ranging from on-chip DNA hybridization up to the detection of antibodies inside diluted human blood serum, will be demonstrated.

Paper Details

Date Published: 26 May 2009
PDF: 9 pages
Proc. SPIE 7365, Bioengineered and Bioinspired Systems IV, 73650Q (26 May 2009); doi: 10.1117/12.821467
Show Author Affiliations
F. Sonntag, Fraunhofer Institut für Werkstoff- und Strahltechnik (Germany)
S. Schmieder, Fraunhofer Institut für Werkstoff- und Strahltechnik (Germany)
N. Danz, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
M. Mertig, Technische Univ. Dresden (Germany)
N. Schilling, Fraunhofer Institut für Werkstoff- und Strahltechnik (Germany)
U. Klotzbach, Fraunhofer Institut für Werkstoff- und Strahltechnik (Germany)
E. Beyer, Fraunhofer Institut für Werkstoff- und Strahltechnik (Germany)


Published in SPIE Proceedings Vol. 7365:
Bioengineered and Bioinspired Systems IV
Ángel B. Rodríguez-Vázquez, Editor(s)

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