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Proceedings Paper

The repair of low dose UV light-induced damage to human skin DNA in condition of trace amount Mg2+
Author(s): Fang Gao; Zhouyi Guo; Changchun Zheng; Rui Wang; Zhiming Liu; Pei Meng; Juan Zhai
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Paper Abstract

Ultraviolet light-induced damage to human skin DNA was widely investigated. The primary mechanism of this damage contributed to form cyclobutane pyrimidine dimmers (CPDs). Although the distribution of UV light-induced CPDs within a defined sequence is similar, the damage in cellular environment which shields the nuclear DNA was higher than that in organism in apparent dose. So we use low UVB light as main study agent. Low dose UV-irradiated HDF-a cells (Human Dermal Fibroblasts-adult cells) which is weaker than epidermic cells were cultured with DMEM at different trace amount of Mg2+ (0mmol/L , 0.1mmol/L , 0.2mmol/L, 0.4mmol/L, 0.8mmol/L, 1.2mmol/L) free-serum DMEM and the repair of DNA strands injured were observed. Treat these cells with DNA strand breaks detection, photoproducts detection and the repair of photoproducts detection. Then quantitate the role of trace amount Mg2+ in repair of UV light-induced damage to human skin. The experiment results indicated that epidermic cells have capability of resistance to UV-radiation at a certain extent. And Mg2+ can regulate the UV-induced damage repair and relative vitality. It can offer a rationale and experiment data to relieve UV light-induced skin disease.

Paper Details

Date Published: 6 March 2009
PDF: 9 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800L (6 March 2009); doi: 10.1117/12.821458
Show Author Affiliations
Fang Gao, South China Normal Univ. (China)
Zhouyi Guo, South China Normal Univ. (China)
Changchun Zheng, South China Normal Univ. (China)
Rui Wang, South China Normal Univ. (China)
Zhiming Liu, South China Normal Univ. (China)
Pei Meng, South China Normal Univ. (China)
Juan Zhai, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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