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Proceedings Paper

Analysis of relationship between apoptosis and change of Ca2+ in HepG-2 induced by CSA with laser scanning confocal technology
Author(s): Yu-bin Ji; Lei Yu
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Paper Abstract

Laser scanning confoncal technology was used to study relationship between apoptosis and change of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effect of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation of HepG-2 cells was completed by fluorescence microscope. Apoptosis induced by CSA on HepG-2 cells was measured by flowcytometry. In addition, change of intracellular Ca2+ level of CSA on HepG-2 cells was observed by laser scanning confocal microscope. As a result, CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50 was 162.4μg/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA. Concentration of Ca2+ in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA had obvious effect of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca2+ might be invovled in these events.

Paper Details

Date Published: 6 March 2009
PDF: 6 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800T (6 March 2009); doi: 10.1117/12.821311
Show Author Affiliations
Yu-bin Ji, Harbin Univ. of Commerce (China)
Engineering Research Ctr. of Natural Anticancer Drugs (China)
Lei Yu, Harbin Univ. of Commerce (China)
Engineering Research Ctr. of Natural Anticancer Drugs (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine

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