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Proceedings Paper

The effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells
Author(s): YuBin Ji; ShiYong Gao; ChenFeng Ji; Xiang Zou
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Paper Abstract

To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+]i in the cells are observed using LCSM. The results of double staining with TMRE and Fluo-3/AM show that solanine can lower membrane potential and increase the concentration of Ca2+ in the cells Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca2+ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca2+ in the cell, turning on the mechanism for apoptosis.

Paper Details

Date Published: 6 March 2009
PDF: 6 pages
Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800Q (6 March 2009); doi: 10.1117/12.821308
Show Author Affiliations
YuBin Ji, Engineering Research Ctr. of Natural Antineoplastic Drugs (China)
Harbin Univ. of Commerce (China)
ShiYong Gao, Engineering Research Ctr. of Natural Antineoplastic Drugs (China)
ChenFeng Ji, Engineering Research Ctr. of Natural Antineoplastic Drugs (China)
Harbin Univ. of Commerce (China)
Xiang Zou, Harbin Univ. of Commerce (China)


Published in SPIE Proceedings Vol. 7280:
Seventh International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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