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Proceedings Paper

Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy
Author(s): Silvia Soria; Franco Quercioli; Raffaella Mercatelli; Federica Bianco; Ilaria Cacciari; Stefano Pelli; Giancarlo Righini
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Paper Abstract

We report on the application of a simple white light source based on the supercontinuum generation from commercial photonic crystal fibres to confocal fluorescence microscopy and fluorescence lifetime imaging (FLIM) microscopy. The coherent white light can be tuned by varying the wavelength and intensity of the pump, a Ti:Sapphire laser. There are several advantages jn the use of SC sources: spatially coherent white radiation, tuning ranges of approximately 400 nm, high brightness, a robust compact system (potentially all-fibre) and relatively low cost. Being pulsed, SC sources are suitable for FLIM and may be used for multiple excitation.

Paper Details

Date Published: 20 May 2009
PDF: 7 pages
Proc. SPIE 7366, Photonic Materials, Devices, and Applications III, 73660R (20 May 2009); doi: 10.1117/12.821199
Show Author Affiliations
Silvia Soria, Centro Studi e Ricerche Enrico Fermi (Italy)
Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Franco Quercioli, Istituto dei Sistemi Complessi, CNR (Italy)
Raffaella Mercatelli, Istituto dei Sistemi Complessi, CNR (Italy)
Federica Bianco, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Ilaria Cacciari, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Stefano Pelli, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)
Giancarlo Righini, Istituto di Fisica Applicata Nello Carrara, CNR (Italy)


Published in SPIE Proceedings Vol. 7366:
Photonic Materials, Devices, and Applications III
Ali Serpenguzel; Gonçal Badenes; Giancarlo C. Righini, Editor(s)

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