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Proceedings Paper

Harmonic holography for three-dimensional cellular imaging
Author(s): Chia-Lung Hsieh; Rachel Grange; Ye Pu; Demetri Psaltis
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Paper Abstract

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from BaTiO3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H2) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H2 microscope.

Paper Details

Date Published: 6 May 2009
PDF: 8 pages
Proc. SPIE 7329, Three-Dimensional Imaging, Visualization, and Display 2009, 73290H (6 May 2009); doi: 10.1117/12.820801
Show Author Affiliations
Chia-Lung Hsieh, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
California Institute of Technology (United States)
Rachel Grange, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Ye Pu, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
California Institute of Technology (United States)
Demetri Psaltis, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
California Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7329:
Three-Dimensional Imaging, Visualization, and Display 2009
Bahram Javidi; Jung-Young Son; Manuel Martinez-Corral; Fumio Okano; Wolfgang Osten, Editor(s)

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