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Proceedings Paper

Development of a real-time system of monitoring bacterial colony growth and registering the forward-scattering pattern
Author(s): Nan Bai; Euiwon Bae; Amornrat Aroonnual; Arun K. Bhunia; J. Paul Robinson; E. Daniel Hirleman
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Paper Abstract

Early detection and classification of pathogenic bacteria species is crucial to food safety. The previous BARDOT (BActeria Rapid Detection by using Optical light scattering Technology) system is capable of classifying the bacterial colonies of around 1~1.5mm diameter within 24~36 hours of incubation. However, in order to further reduce the detection time and synchronize the detection operation with the bacterial cultivation, a micro-incubator is developed that not only grows bacteria at 37°C but also enables forward scatterometry. This new design feature enables us to continuously characterize the light scattering patterns of the bacterial colonies throughout their growing stages. Some experimental results from this new system are demonstrated and compared with the images obtained from phase contrast microscopy and a confocal displacement meter to show the possibility of earlier identification of bacteria species. Moreover, this paper also explains the updated optical and mechanical modules for the beam waist control to accommodate the smaller bacteria colony detection.

Paper Details

Date Published: 27 April 2009
PDF: 8 pages
Proc. SPIE 7315, Sensing for Agriculture and Food Quality and Safety, 73150Z (27 April 2009); doi: 10.1117/12.818961
Show Author Affiliations
Nan Bai, Purdue Univ. (United States)
Euiwon Bae, Purdue Univ. (United States)
Amornrat Aroonnual, Purdue Univ. (United States)
Arun K. Bhunia, Purdue Univ. (United States)
J. Paul Robinson, Purdue Univ. (United States)
E. Daniel Hirleman, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 7315:
Sensing for Agriculture and Food Quality and Safety
Moon S. Kim; Shu-I Tu; Kaunglin Chao, Editor(s)

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