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Proceedings Paper

Label-free DNA methylation analysis using the optofluidic ring resonator sensor
Author(s): Jonathan D. Suter; Daniel J. Howard; Charles W. Caldwell; Huidong Shi; Xudong Fan
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Paper Abstract

We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for the purpose of analyzing the degree of methylation in sample oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, is prone to abnormal behavior that may lead to uncontrolled suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool to explore and monitor the degree of methylation in DNA. Two different approaches are explored, using either bisulfite modification or immunoprecipitation. The methods are compared and the signal response for both methods is characterized.

Paper Details

Date Published: 29 April 2009
PDF: 8 pages
Proc. SPIE 7322, Photonic Microdevices/Microstructures for Sensing, 732208 (29 April 2009); doi: 10.1117/12.818562
Show Author Affiliations
Jonathan D. Suter, Univ. of Missouri, Columbia (United States)
Daniel J. Howard, Univ. of Missouri, Columbia (United States)
Charles W. Caldwell, Univ. of Missouri, Columbia (United States)
Huidong Shi, Univ. of Missouri, Columbia (United States)
Medical College of Georgia Cancer Ctr. (United States)
Xudong Fan, Univ. of Missouri, Columbia (United States)

Published in SPIE Proceedings Vol. 7322:
Photonic Microdevices/Microstructures for Sensing
Hai Xiao; Xudong Fan; Anbo Wang, Editor(s)

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