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Proceedings Paper

Time-resolved FRET for single-nucleotide polymorphism genotyping
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Paper Abstract

By tens-of-picosecond resolved fluorescence detection (TCSPC, time-correlated single-photon counting) we study Förster resonance energy transfer between a donor and a black-hole-quencher acceptor bound at the 5'- and 3'-positions of a synthetic DNA oligonucleotide. This dual labelled oligonucleotide is annealed with either the complementary sequence or with sequences that mimic single-nucleotide polymorphic gene sequences: they differ in one nucleotide at positions near either the ends or the center of the oligonucleotide. We find donor fluorescence decay times whose values are definitely distinct and discuss the feasibility of single nucleotide polymorphism genotyping by this method.

Paper Details

Date Published: 29 April 2009
PDF: 7 pages
Proc. SPIE 7320, Advanced Photon Counting Techniques III, 732014 (29 April 2009); doi: 10.1117/12.818461
Show Author Affiliations
Alessandra Andreoni, Univ. degli Studi dell'Insubria (Italy)
CNISM (Italy)
Luca Nardo, Univ. degli Studi dell'Insubria (Italy)
CNISM (Italy)
Maria Bondani, National Lab. for Ultrafast and Ultraintense Optical Science, INFM (Italy)

Published in SPIE Proceedings Vol. 7320:
Advanced Photon Counting Techniques III
Mark A. Itzler; Joe C. Campbell, Editor(s)

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