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Proceedings Paper

Antibody-functionalized magnetic nanowires for cell purification
Author(s): Ning Gao; Xiaochuan Yang; Yao-Tsan Tsai; Geh Meh Chu; Hongjun Wang; Eui-Hyeok Yang
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Paper Abstract

Rapid and efficient cell purification remains challenging. The use of ferromagnetic Ni nanowires for cell purification is considered superior over magnetic beads. In this study, we explored the opportunity to improve cell purification by using antibody-functionalized Ni nanowires. Antibody (anti-CD31) against mouse endothelial cells (MS-1) was conjugated to Ni nanowire surface by self-assembled monolayers (SAMs) and chemical covalent reaction. The antibody functionalized nanowires were used to purify the MS-1 from a mixture of MS-1 and mouse fibroblast cells (3T3). The nanowire-bound cells were magnetically separated to determine the separation yield of target cells. Furthermore, the proliferation of nanowire-bound cells was studied by MTT cell proliferation assay. This work demonstrates that antibody-functionalized Ni nanowires provide an effective mean to separate cells.

Paper Details

Date Published: 11 May 2009
PDF: 6 pages
Proc. SPIE 7318, Micro- and Nanotechnology Sensors, Systems, and Applications, 73181E (11 May 2009); doi: 10.1117/12.818402
Show Author Affiliations
Ning Gao, Stevens Institute of Technology (United States)
Xiaochuan Yang, Stevens Institute of Technology (United States)
Yao-Tsan Tsai, Stevens Institute of Technology (United States)
Geh Meh Chu, Stevens Institute of Technology (United States)
Hongjun Wang, Stevens Institute of Technology (United States)
Eui-Hyeok Yang, Stevens Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7318:
Micro- and Nanotechnology Sensors, Systems, and Applications
Thomas George; M. Saif Islam; Achyut K. Dutta, Editor(s)

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