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Proceedings Paper

Applications of spectral imaging using a tunable laser source
Author(s): David C. Oertel; Jeffrey T. Grothaus; Curtis Marcott
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Paper Abstract

Hyperspectral reflectance imaging in the visible and NIR spectral ranges has considerable utility for revealing spatial and chemical complexity in both biological systems and manufactured products. Conventional imaging systems are based on broad-band illumination in tandem with a spectrometer or tunable filter placed between the sample and the detector. These systems are typically slow (require seconds of integration per wavelength step), and the CW broad-band source can cause significant heating of the sample. An alternative method is to use a tunable, pulsed, high-peak-power (low average power) source coupled with a broad-band detector. This approach offers a reduction in data acquisition time, the inherent ability to stop motion, and data collection at ambient temperature. An integrated system based on a 5- ns pulsed laser tunable from 430 nm to 2150 nm has been used to obtain hyperspectral images in both the visible and NIR spectral ranges. A number of camera/lens options allow for varied spectral bandwidths and the FOV, ranging from 11 × 15 mm2 to 15 × 20 cm2. An entire hyperspectral image stack can be collected in as little as 20 s. This method, allowing fast, room-temperature data acquisition, has sufficient sensitivity to produce data that can be successfully processed using spectral derivatives and multivariate analysis. We discuss several applications, both in vivo and otherwise, of this alternative approach to visible/NIR hyperspectral imaging.

Paper Details

Date Published: 29 April 2009
PDF: 8 pages
Proc. SPIE 7319, Next-Generation Spectroscopic Technologies II, 731906 (29 April 2009); doi: 10.1117/12.818147
Show Author Affiliations
David C. Oertel, The Procter & Gamble Co. (United States)
Jeffrey T. Grothaus, The Procter & Gamble Co. (United States)
Curtis Marcott, Light Light Solutions, LLC (United States)


Published in SPIE Proceedings Vol. 7319:
Next-Generation Spectroscopic Technologies II
Mark A. Druy; Christopher D. Brown; Richard A. Crocombe, Editor(s)

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