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Proceedings Paper

3D microscopy with a double-helix point spread function
Author(s): Sri Rama Prasanna Pavani; Rafael Piestun
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Paper Abstract

Double-helix point spread function (DH-PSF) is an engineered three-dimensional (3D) PSF specifically designed for 3D position estimation and imaging. It exhibits two lobes that rotate continuously around the optical axis with propagation. An information theoretical analysis shows that the DH-PSF carries higher and more uniform Fisher Information than the standard PSF through the 3D volume of interest. Experiments with DH-PSF demonstrate nanometer scale position localization accuracies in all three dimensions. Further, a variety of microscopy techniques such as bright-field, dark-field, and fluorescence can be directly transformed into their DH-PSF counterparts by placing an appropriate phase mask in the imaging path. The flexibility and performance make the DH-PSF attractive for 3D position localization in both photon-limited and photon-unlimited regimes.

Paper Details

Date Published: 3 March 2009
PDF: 7 pages
Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 71840I (3 March 2009); doi: 10.1117/12.816585
Show Author Affiliations
Sri Rama Prasanna Pavani, Univ. of Colorado at Boulder (United States)
Rafael Piestun, Univ. of Colorado at Boulder (United States)


Published in SPIE Proceedings Vol. 7184:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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