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Proceedings Paper

Meeting current public health needs: optical biosensors for pathogen detection and analysis
Author(s): Minghui Yang; Kim E Sapsford; Nikolay Sergeev; Steven Sun; Avraham Rasooly
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Paper Abstract

Pathogen detection and analysis is critical for medicine, food safety, agriculture, public health and biosecurity. Many current microbial detection approaches are based on century-old culturing methods which, while reliable, are slow, provide relatively little information about the pathogens and are not adaptable to high throughput operations. Optical biodetection represents a potential alternative. Most ELISA and chromatography systems are based on optical methods that are also used for analysis of molecular interactions, such as DNA hybridization and protein-protein interactions (e.g. microarrays or SPR biosensors). Various optical biosensor platforms have been developed that have many of the characteristics essential for modern pathogen molecular analysis including sensitivity, speed of analysis, multi-channel capability, relative simplicity and low cost. Here we provide several examples of the use of optical biosensor technology for pathogen detection and analysis including high throughput DNA microarray analysis, SPR-based rapid direct detection of bacterial toxins, CCD-based fluorescent activity analysis of microbial toxins and a simple ECL-based CCD detection system. However, while effective for molecular analysis, most of these technologies are not as sensitive as traditional culturing methods for detecting microorganisms. There is a need to combine optical biosensors with traditional methods to speed culture-based detection and to provide more information regarding the pathogens.

Paper Details

Date Published: 19 February 2009
PDF: 14 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 716702 (19 February 2009); doi: 10.1117/12.816464
Show Author Affiliations
Minghui Yang, University of Maryland, Baltimore County (United States)
Kim E Sapsford, FDA (United States)
Nikolay Sergeev, FDA (United States)
Steven Sun, University of Maryland, Baltimore County (United States)
Avraham Rasooly, FDA (United States)
National Cancer Institute (United States)

Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

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