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Proceedings Paper

Laser-scanning optical-resolution photoacoustic microscopy
Author(s): Hao F. Zhang; Zhixing Xie; Shuliang Jiao; Carmen A Puliafito
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Paper Abstract

We have developed a laser-scanning optical-resolution photoacoustic microscopy that can potentially be easily integrated with several existing optical microscopic modalities. During data acquisition, the ultrasonic transducer is kept stationary and only the laser light is raster-scanned by an x-y galvanometer scanner. In this configuration, the field-ofview is limited by the beam diameter of the ultrasonic detector, which is related to the active element size, numerical aperture, and the center frequency of the ultrasonic transducer. The spatial resolution is determined by the size of the optical focus. A lateral resolution of 7.8 μm and a circular field-of-view with a diameter of 6 mm were achieved in an optically clear medium. Using a laser system working at a pulse repetition rate of 1024 Hz, the data acquisition time for an image consisting of 256×256 pixels was less than two minutes. In vivo imaging of microvasculature in mouse ears were also achieved.

Paper Details

Date Published: 24 February 2009
PDF: 6 pages
Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71770L (24 February 2009); doi: 10.1117/12.815396
Show Author Affiliations
Hao F. Zhang, Univ. of Wisconsin, Milwaukee (United States)
Zhixing Xie, Univ. of Wisconsin, Milwaukee (United States)
Shuliang Jiao, Univ. of Southern California (United States)
Carmen A Puliafito, Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 7177:
Photons Plus Ultrasound: Imaging and Sensing 2009
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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