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Proceedings Paper

Multicolor fluctuation spectroscopy in cells
Author(s): Michelle A. Digman; Enrico Gratton
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Paper Abstract

Based on the Fluctuation Correlation principle we have developed a method that is capable of measuring the stoichiometry of molecular complexes and mapping dynamic processes in living cells. The method is based on measuring simultaneously fluctuations of the fluorescence intensity at two image channels, each detecting a different kind of protein. This is an extension of the number and brightness analysis in which we use the use of the ratio between the variance and the average intensity to obtain the brightness of molecules.

Paper Details

Date Published: 13 February 2009
PDF: 10 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718303 (13 February 2009); doi: 10.1117/12.814875
Show Author Affiliations
Michelle A. Digman, Univ. of California, Irvine (United States)
Enrico Gratton, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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