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Proceedings Paper

Multimodal three-dimensional imaging with isometric high resolution using optical projection tomography
Author(s): Qin Miao; J. Richard Rahn; Ryland C. Bryant; Christy A. Lancaster; Anna Tourovskaia; Thomas Neumann; Eric J. Seibel; Alan C. Nelson
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Paper Abstract

The optical projection tomography microscope (OPTM) is an optical microscope that acquires focus-invariant images from multiple views of single cells. Although the depth of field of the objective is short, it can be extended by scanning the objective's focal plane. This extended depth of field image is similar to a projection in conventional X-ray CT. Samples flow through a microcapillary tube filled with optical gel. Optical distortion is minimized by matching refractive index of optical gel and tube. Multiple projection images are taken by rotating the microcapillary tube with sub-micron mechanical precision. After these pseudoprojection images are further aligned, computed tomography methods are then applied to the images to create a 3D reconstruction with isometric resolution of 0.35 microns. Three-dimensional reconstructed images of fluorescent microspheres and cells are shown.

Paper Details

Date Published: 28 February 2009
PDF: 8 pages
Proc. SPIE 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging, 72620V (28 February 2009); doi: 10.1117/12.813844
Show Author Affiliations
Qin Miao, Univ. of Washington (United States)
J. Richard Rahn, Univ. of Washington (United States)
VisionGate, Inc. (United States)
Ryland C. Bryant, Univ. of Washington (United States)
VisionGate, Inc. (United States)
Christy A. Lancaster, Univ. of Washington (United States)
VisionGate, Inc. (United States)
Anna Tourovskaia, Univ. of Washington (United States)
VisionGate, Inc. (United States)
Thomas Neumann, Univ. of Washington (United States)
VisionGate, Inc. (United States)
Eric J. Seibel, Univ. of Washington (United States)
Alan C. Nelson, Univ. of Washington (United States)
VisionGate, Inc. (United States)


Published in SPIE Proceedings Vol. 7262:
Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging
Xiaoping P. Hu; Anne V. Clough, Editor(s)

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