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Proceedings Paper

Live TIRF microscopy at 100nm resolution through structured illumination
Author(s): P. Kner; B. Chhun; E. Griffis; L. Winoto; L. Shao; M. G. L. Gustafsson
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Paper Abstract

Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.

Paper Details

Date Published: 4 March 2009
PDF: 9 pages
Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 718417 (4 March 2009); doi: 10.1117/12.812351
Show Author Affiliations
P. Kner, Univ. of California, San Francisco (United States)
B. Chhun, Univ. of California, San Francisco (United States)
E. Griffis, Univ. of California, San Francisco (United States)
L. Winoto, Univ. of California, San Francisco (United States)
L. Shao, Univ. of California, San Francisco (United States)
M. G. L. Gustafsson, Univ. of California, San Francisco (United States)


Published in SPIE Proceedings Vol. 7184:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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