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Proceedings Paper

Raman spectroscopy and rotating orthogonal polarization imaging for non-destructive tracking of collagen deposition in tissue engineered skin and tendon
Author(s): Melissa L. Mather; Steve P. Morgan; David E. Morris; Qun Zhu; Jasmin Kee; Alina Zoladek; John A. Crowe; Ioan Notingher; David J. Williams; Penny A. Johnson
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Paper Abstract

To ensure the sustainability of tissue engineered products there is a need to consider the engineering and manufacturing issues related to them particularly for the purposes of process optimization and product quality assurance. This work describes the application of Raman spectroscopy for in process monitoring of a skin substitute and rotating orthogonal polarization imaging to track collagen alignment in a tissue engineered tendon. The skin substitute studied is produced from culturing fibroblasts in a fibrin matrix. Throughout the production process the fibroblasts secrete extracellular matrix and in doing so deposit collagen in the matrix. Key to optimization of the skin substitute production process is development of strategies to track the collagen and fibrin content. The work presented here discusses the feasibility of Raman spectroscopy to resolve fibrin and collagen components in the skin substitutes. Collagen alignment is also important in the engineering of many tissues, in particular tendons. Thus, this work will also investigate the ability of rotating orthogonal polarization imaging to track collagen alignment in a tissue engineered tendon.

Paper Details

Date Published: 23 February 2009
PDF: 10 pages
Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790A (23 February 2009); doi: 10.1117/12.812221
Show Author Affiliations
Melissa L. Mather, Univ. of Nottingham (United Kingdom)
Steve P. Morgan, Univ. of Nottingham (United Kingdom)
David E. Morris, Univ. of Nottingham (United Kingdom)
Qun Zhu, Univ. of Nottingham (United Kingdom)
Jasmin Kee, Loughborough Univ. (United Kingdom)
Alina Zoladek, Univ. of Nottingham (United Kingdom)
John A. Crowe, Univ. of Nottingham (United Kingdom)
Ioan Notingher, Univ. of Nottingham (United Kingdom)
David J. Williams, Loughborough Univ. (United Kingdom)
Penny A. Johnson, Intercytex (United Kingdom)


Published in SPIE Proceedings Vol. 7179:
Optics in Tissue Engineering and Regenerative Medicine III
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)

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