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Proceedings Paper

Real-time optical imaging of the interaction of epidermal growth factor and its receptor in living cells
Author(s): Qiaoya Lin; Liang Wang; Shaoqun Zeng; Zhihong Zhang; Gang Zheng
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Paper Abstract

Fluorescence resonance energy transfer (FRET) has been widely used in biology in recent years, and permits high spatial resolution assays of protein-protein interactions in living cells. Here, we first use the FRET technique to real-time observe the binding of EGF to EGFR on the surface of A549 cells and EGFR-GFP-ldlA7 cells, and continuously monitor this reaction for 1 hour. In addition, this is the first direct evidence that FRET occurred between different proteins which are in the intramembrane and extramembrane, respectively.

Paper Details

Date Published: 24 February 2009
PDF: 7 pages
Proc. SPIE 7178, Biophotonics and Immune Responses IV, 71780P (24 February 2009); doi: 10.1117/12.811372
Show Author Affiliations
Qiaoya Lin, Huazhong Univ. of Science and Technology (China)
Liang Wang, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)
Gang Zheng, Ontario Cancer Institute (Canada)
Univ. of Toronto (Canada)

Published in SPIE Proceedings Vol. 7178:
Biophotonics and Immune Responses IV
Wei R. Chen, Editor(s)

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