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Proceedings Paper

Implantable optical biosensor for in vivo molecular imaging
Author(s): Thomas D. O'Sullivan; Elizabeth Munro; Adam de la Zerda; Natesh Parashurama; Robert Teed; Zachary Walls; Ofer Levi; Sanjiv S. Gambhir; James S. Harris
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Paper Abstract

We present the design and fabrication of an implantable fluorescence biosensor suitable for continuously monitored, freely-moving in vivo rodent studies. The GaAs-based semiconductor sensor incorporates an un-cooled photodetector with a 670nm vertical-cavity surface-emitting laser (VCSEL) optimized for sensing fluorescent Cy5.5 dye. For filtering unwanted spectra, a combination of physical and spectral blocking layers yields OD5 excitation rejection at the detector. The sensor detects near-IR fluorescent Cy5.5 molecules in vitro at 100nM concentration (in a 100μL volume) with linear response for concentrations up to 25μM. In a preliminary study in a living mouse, subcutaneously injected dye (1μM Cy5.5 in 50μL) was detected. This technology has the potential to enable new studies of living systems in applications that require long-term, continuous fluorescence sensing.

Paper Details

Date Published: 20 February 2009
PDF: 8 pages
Proc. SPIE 7173, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX, 717309 (20 February 2009); doi: 10.1117/12.811227
Show Author Affiliations
Thomas D. O'Sullivan, Stanford Univ. (United States)
Elizabeth Munro, Univ. of Toronto (Canada)
Adam de la Zerda, Stanford Univ. (United States)
Natesh Parashurama, Stanford Univ. (United States)
Robert Teed, Stanford Univ. (United States)
Zachary Walls, Stanford Univ. (United States)
Ofer Levi, Univ. of Toronto (Canada)
Sanjiv S. Gambhir, Stanford Univ. (United States)
James S. Harris, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 7173:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications IX
Israel Gannot, Editor(s)

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