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Proceedings Paper

Wide horizons for optical trapping: simultaneous optical manipulation and confocal imaging of live T cells at low magnification
Author(s): J. Harris; G. McConnell
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Paper Abstract

We report a system for optical trapping, manipulation and imaging of live cells in close to real-time with a wide field of view. A fs-pulsed Ti:Sapphire source was used to trap cells whilst an Ar+ laser was used for confocal imaging. A single 10x/0.4NA lens and a dual scanning system enabled independent trapping, imaging and movement of cells over a field of view >1mm2. We will present movies of controlled T cell and dendritic cell movement and interaction. This enables the functional study of cell-signalling processes that are fundamental to understanding the behaviour of the immune system and beyond.

Paper Details

Date Published: 28 February 2009
PDF: 13 pages
Proc. SPIE 7193, Solid State Lasers XVIII: Technology and Devices, 719327 (28 February 2009); doi: 10.1117/12.811160
Show Author Affiliations
J. Harris, Univ. of Strathclyde (United Kingdom)
G. McConnell, Univ. of Strathclyde (United Kingdom)


Published in SPIE Proceedings Vol. 7193:
Solid State Lasers XVIII: Technology and Devices
W. Andrew Clarkson; Norman Hodgson; Ramesh K. Shori, Editor(s)

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