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Proceedings Paper

Spiropyrans as molecular optical switches
Author(s): B. Seefeldt; R. Kasper; M. Beining; K. Altenhöner; O. Tosic; J. Arden-Jacob; N. Kemnitzer; J. Mattay; K.-H. Drexhage; M. Heilemann; M. Sauer
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Paper Abstract

Photochromic molecules that can be switched between a fluorescent on-state and a nonfluorescent off-state are a key element of modern high-resolution microscopy methods, such as STORM (stochastic optical resolution microscopy, [1]) and RESOLFT (reversible saturable optical fluorescence transitions [2]) The applicability of molecular optical switches (photoswitches) for superresolution fluorescence microscopy methods depends on various features, i.e. (i) reversible photoswitching (ideally by using visible light), (ii) sufficient thermal stability in both states, (iii) proper switching rates, (iv) a high photon yield in the fluorescent state and a (v) nondestructive readout-capability. Important for biological applications are solubility in water and a maintained switching capability in aqueous surrounding. Today, only a small number of organic photoswitches are known, such as Cy5, Cy 7 and Alexa647, and Alexa680 [3, 19, 21]. Here, we present our approach to design new photoswitches. We introduce Spiropyran derivatives which either fluoresce on their own or are conjugated to an organic fluorophore and switch its fluorescence via fluorescence resonance energy transfer (FRET) [4]. We present ensemble data of the switching process of various spiropyrans and spiropyran-dye-conjugates as well as the documentation of the switching process at the single-molecule level. Furthermore, we present a water soluble spiropyran derivative that is switchable even in aqueous surrounding.

Paper Details

Date Published:
Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850L; doi: 10.1117/12.810700
Show Author Affiliations
B. Seefeldt, Bielefeld Univ. (Germany)
R. Kasper, Bielefeld Univ. (Germany)
M. Beining, Bielefeld Univ. (Germany)
K. Altenhöner, Bielefeld Univ. (Germany)
O. Tosic, Bielefeld Univ. (Germany)
J. Arden-Jacob, Siegen Univ. (Germany)
N. Kemnitzer, Siegen Univ. (Germany)
J. Mattay, Bielefeld Univ. (Germany)
K.-H. Drexhage, Siegen Univ. (Germany)
M. Heilemann, Bielefeld Univ. (Germany)
M. Sauer, Bielefeld Univ. (Germany)

Published in SPIE Proceedings Vol. 7185:
Single Molecule Spectroscopy and Imaging II
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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