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Proceedings Paper

Metabolic measurements in cell culture and tissue constructs
Author(s): P. Rolfe
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Paper Abstract

This paper concerns the study and use of biological cells in which there is a need for sensors and assemblies for the measurement of a diverse range of physical and chemical variables. In this field cell culture is used for basic research and for applications such as protein and drug synthesis, and in cell, tissue and organ engineering. Metabolic processes are fundamental to cell behaviour and must therefore be monitored reliably. Basic metabolic studies measure the transport of oxygen, glucose, carbon dioxide, lactic acid to, from, or within cells, whilst more advanced research requires examination of energy storage and utilisation. Assemblies are designed to incorporate bioreactor functions for cell culture together with appropriate sensing devices. Oxygen consumption by populations of cells is achieved in a flowthrough assembly that incorporates O2 micro-sensors based on either amperometry or fluorescence. Measurements in single cell are possible with intra-cellular fluorophores acting as biosensors together with optical stimulation and detection. Near infra-red spectroscopy (NIRS) is used for analysis within culture fluid, for example for estimation of glucose levels, as well as within cell populations, for example to study the respiratory enzymes.£

Paper Details

Date Published: 12 January 2009
PDF: 8 pages
Proc. SPIE 7133, Fifth International Symposium on Instrumentation Science and Technology, 713318 (12 January 2009); doi: 10.1117/12.810606
Show Author Affiliations
P. Rolfe, Oxford BioHorizons Ltd. (United Kingdom)
Harbin Institute of Technology (China)
Waseda Univ. (Japan)


Published in SPIE Proceedings Vol. 7133:
Fifth International Symposium on Instrumentation Science and Technology
Jiubin Tan; Xianfang Wen, Editor(s)

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