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Proceedings Paper

Comparison of two-photon imaging depths with 775 nm excitation and 1300 nm excitation
Author(s): Demirhan Kobat; Angela Wong; Chris B. Schaffer; Chris Xu
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Paper Abstract

We quantitatively compared the maximal two-photon fluorescence microscopy imaging depth achieved with 775 nm excitation to that achieved with 1300 nm excitation through ex-vivo TPM of blood vessels in the mouse brain. We achieved high contrast imaging of labeled blood vessels at approximately twice the depth at 1300 nm excitation as at 775 nm excitation. We also measured the two-photon excitation cross-sections of several commercially available fluorophores at 1220-1320 nm. We found that some of these fluorophores reveal comparable if not better cross-section values than those of the widely used dyes excited by shorter wavelength light.

Paper Details

Date Published: 13 February 2009
PDF: 7 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832D (13 February 2009); doi: 10.1117/12.810506
Show Author Affiliations
Demirhan Kobat, Cornell Univ. (United States)
Angela Wong, Cornell Univ. (United States)
Chris B. Schaffer, Cornell Univ. (United States)
Chris Xu, Cornell Univ. (United States)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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