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Proceedings Paper

Solid-phase purification of gene synthesis products using magnetic beads
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Paper Abstract

Herein we report three novel methods which utilize chemical conjugated magnetic beads to purify synthetic gene from its synthesis solution and prepare the synthesized gene in suitable buffer for downstream applications. Silica-coated magnetic beads are applied for non-specific DNA purification to remove short oligonucleotides and monomers. Streptavidin conjugated magnetic beads and (dT)25 Oligo immobilized magnetic beads are introduced for specific DNA extraction. The performances of these methods are investigated and compared using gel electrophoresis. The optimal conditions for enhancing the extraction efficiency are discussed. In addition, the approach to integrate these solid-phase purification methods into microfluidic devices is presented.

Paper Details

Date Published: 30 December 2008
PDF: 11 pages
Proc. SPIE 7269, Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV, 72690A (30 December 2008); doi: 10.1117/12.810439
Show Author Affiliations
Mo Chao Huang, Institute of Bioengineering and Nanotechnology (Singapore)
Mo-Huang Li, Institute of Bioengineering and Nanotechnology (Singapore)
Jackie Y. Ying, Institute of Bioengineering and Nanotechnology (Singapore)


Published in SPIE Proceedings Vol. 7269:
Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV
Jung-Chih Chiao; Alex J. Hariz; David V. Thiel; Changyi Yang, Editor(s)

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