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Proceedings Paper

Adaptive optics for multiphoton microscopy
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Paper Abstract

Specimen-induced aberrations are frequently encountered in high resolution microscopy, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations distort the focal spot causing a reduction in resolution and, often more importantly, reduced signal level and contrast. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have developed an adaptive two-photon fluorescence microscope to correct for these aberrations. Unlike a conventional adaptive optics system, the microscope does not include a wavefront sensor but uses an efficient sensorless optimisation scheme to obtain optimum aberration correction.

Paper Details

Date Published: 13 February 2009
PDF: 8 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831J (13 February 2009); doi: 10.1117/12.810367
Show Author Affiliations
Delphine Débarre, Univ. of Oxford (United Kingdom)
Tony Wilson, Univ. of Oxford (United Kingdom)
Martin J. Booth, Univ. of Oxford (United Kingdom)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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