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Proceedings Paper

FRET imaging of multiple focal planes to analyze the organization and conformation of transferrin-receptor in polarized cells
Author(s): Horst Wallrabe; Ammasi Periasamy; Margarida Barroso
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Paper Abstract

We developed a FRET-based assay to analyze multiple focal planes from z-staks collected using confocal imaging of polarized epithelial MDCK cells. To establish the imaging assay for multiple focal planes, we have used fixed cells, where changes in the organization and conformation of transferrin-receptor complexes between endosomes are expected to be minimized. Therefore, we indeed only see minor changes in E% throughout the endocytic pathway of polarized cells. In the future, we will apply this FRET-based assay to live polarized epithelial cells to investigate the factors involved in the regulation of the organization and conformation of membrane-bound receptors during endocytic trafficking.

Paper Details

Date Published: 25 February 2009
PDF: 6 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832F (25 February 2009); doi: 10.1117/12.810246
Show Author Affiliations
Horst Wallrabe, Univ. of Virginia (United States)
Ammasi Periasamy, Univ. of Virginia (United States)
Margarida Barroso, Albany Medical College (United States)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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