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Proceedings Paper

Inhibition of yeast growth during long term exposure to laser light around 1064 nm
Author(s): Thomas Aabo; Ivan R. Perch-Nielsen; Jeppe Seidelin Dam; Darwin Z. Palima; Henrik Siegumfeldt; Jesper Glückstad; Nils Arneborg
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Paper Abstract

We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.

Paper Details

Date Published: 6 February 2009
PDF: 7 pages
Proc. SPIE 7227, Complex Light and Optical Forces III, 722706 (6 February 2009); doi: 10.1117/12.810146
Show Author Affiliations
Thomas Aabo, Copenhagen Univ. (Denmark)
Ivan R. Perch-Nielsen, Technical Univ. of Denmark (Denmark)
Jeppe Seidelin Dam, Technical Univ. of Denmark (Denmark)
Darwin Z. Palima, Technical Univ. of Denmark (Denmark)
Henrik Siegumfeldt, Copenhagen Univ. (Denmark)
Jesper Glückstad, Technical Univ. of Denmark (Denmark)
Nils Arneborg, Copenhagen Univ. (Denmark)

Published in SPIE Proceedings Vol. 7227:
Complex Light and Optical Forces III
Enrique J. Galvez; David L. Andrews; Jesper Glückstad, Editor(s)

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