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Proceedings Paper

Continuous-flow PCR using segmented flow and integrating sample preparation
Author(s): Holger Becker; Nadine Hlawatsch; Cornelia Carstens; Richard Klemm; Claudia Gärtner
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Paper Abstract

Continuous-flow PCR has proven to be a powerful method for the amplification of genetic material due to its high speed and the possibility to perform amplicon detection and separation on-chip. A unique possibility of this method is the simultaneous amplification of several samples within a single chip by sample stacking, either having identical samples in several sample plugs separated by e.g. a mineral oil or using different samples in each sample plug. We have demonstrated the viability of sample stacking with a commercially available continuous-flow PCR system with a variety of protocols and samples. Further integration steps like thermal lysis and on-chip lyophilisate storage have been performed, with subsequent successful PCR. Chip modules for DNA extraction either with magnetic beads or membrane filters have been developed.

Paper Details

Date Published: 23 February 2009
PDF: 6 pages
Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070J (23 February 2009); doi: 10.1117/12.809980
Show Author Affiliations
Holger Becker, microfluidic ChipShop GmbH (Germany)
Nadine Hlawatsch, microfluidic ChipShop GmbH (Germany)
Cornelia Carstens, microfluidic ChipShop GmbH (Germany)
Richard Klemm, microfluidic ChipShop GmbH (Germany)
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 7207:
Microfluidics, BioMEMS, and Medical Microsystems VII
Wanjun Wang, Editor(s)

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