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Proceedings Paper

Spectroscopic, cyto-, and photo-toxicity studies of substituted piperidones: potential sensitizers for two-photon photodynamic therapy
Author(s): Kurt W. Short; Tiffany L. Kinnibrugh; David M. Sammeth; Tatiana V. Timofeeva
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Paper Abstract

Two-photon photodynamic therapy has the advantages of being highly localized in its effects and allows for deeper tissue penetration, when compared to one-photon photodynamic therapy. N-alkylated 3,5-bis(arylidene)-4-piperidones, with a donor-pi-acceptor-pi-donor structure, have the potential to be useful two-photon sensitizers. We have measured two-photon cross sections (using femtosecond excitation), fluorescence quantum yields, fluorescence lifetimes, and xray crystal structures for a number of these compounds. Most two-photon cross sections are comparable to or larger than that of Rhodamine B. However, the fluorescence quantum yields are low (all less than 10%) and the fluorescence lifetimes are less than 1 ns (with one exception), suggesting that there may be a significant energy transfer to the triplet state. This would encourage singlet oxygen formation and increase cellular toxicity. Results of dark cyto-toxicity studies with several human cancer cell lines are presented. White light photo-toxicity results are also presented, and suggest that increasing the number of double bonds, from one to two, in the piperidone wings increases the photo-toxicity with little corresponding change in the dark cyto-toxicity.

Paper Details

Date Published: 18 February 2009
PDF: 9 pages
Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 716411 (18 February 2009); doi: 10.1117/12.809904
Show Author Affiliations
Kurt W. Short, New Mexico Highlands Univ. (United States)
Tiffany L. Kinnibrugh, New Mexico Highlands Univ. (United States)
David M. Sammeth, New Mexico Highlands Univ. (United States)
Tatiana V. Timofeeva, New Mexico Highlands Univ. (United States)


Published in SPIE Proceedings Vol. 7164:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII
David H. Kessel, Editor(s)

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