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Proceedings Paper

Ultrasound-modulated fluorescence based on a fluorophore-quencher labeled microbubble system
Author(s): Baohong Yuan
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Paper Abstract

Ultrasound-modulated fluorescence from a fluorophore-quencher labeled microbubble system driven by a single ultrasound pulse was theoretically quantified by solving a modified Herring equation (for bubble oscillation), a two-energy-level rate equation (for fluorophore excitation), and a diffusion equation (for light propagation in tissue). The efficiency of quenching caused by fluorescence resonance energy transfer (FRET) between the fluorophore and the quencher was modulated when the microbubble oscillates in size driven by the ultrasound pulse. Both intensity- and lifetime-based imaging methods are discussed. An important finding in this study is that ultrasound-modulated fluorescent photons may be temporally separated from the un-modulated fluorescent photons if a super-short laser pulse is adopted. This result implies that the modulation efficiency may only be limited by thermal noise of the measurement system.

Paper Details

Date Published: 24 February 2009
PDF: 10 pages
Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71771J (24 February 2009); doi: 10.1117/12.809847
Show Author Affiliations
Baohong Yuan, The Catholic Univ. of America (United States)

Published in SPIE Proceedings Vol. 7177:
Photons Plus Ultrasound: Imaging and Sensing 2009
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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