Share Email Print
cover

Proceedings Paper

Metabolic mapping of cell culture growth by NADH fluorescence lifetime imaging
Author(s): Vladimir Ghukasyan; Tatyana Buryakina; Fu-Jen Kao
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) has been demonstrated as advantageous at discrimination between free and protein-bound forms of the NADH coenzyme, providing not only with the lifetimes of the both states (shorter τ1 and longer τ2), but also with the relative concentrations of both (fractions α1 and α2 correspondingly). Given the role of NADH in cellular energetics, NADH FLIM has been applied for the noninvasive characterization of metabolic changes in a range of pathologies. However, for the discrimination of pathological states, a proper characterization of NADH fluorescence lifetime dynamics at physiological conditions has to be conducted. We have applied FLIM NADH for the characterization of metabolic changes during cell culture growth. Our results demonstrate that during the exponential growth stage there's a well expressed trends of gradual decrease of the free/bound ratio, as measured from the center from the cell colonies. At the same time the cells at the edges of a colony exhibit higher values of the ratio. Several possible reasons for the phenomena observed are discussed.

Paper Details

Date Published: 13 February 2009
PDF: 8 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718309 (13 February 2009); doi: 10.1117/12.809840
Show Author Affiliations
Vladimir Ghukasyan, National Yang-Ming Univ. (Taiwan)
Tatyana Buryakina, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)


Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top