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Proceedings Paper

Role of ROS-mediated TGF beta activation in laser photobiomodulation
Author(s): Praveen R. Arany; Aaron Chih-Hao Chen; Tristan Hunt; David J. Mooney; Michael Hamblin
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Paper Abstract

The ability of laser light to modulate specific biological processes has been well documented but the precise mechanism mediating these photobiological interactions remains an area of intense investigation. We recently published the results of our clinical trial with 30 patients in an oral tooth-extraction wound healing model using a 904nm GaAs laser (Oralaser 1010, Oralia, Konstnaz, Germany), assessing healing parameters using routine histopathology and immunostaining (Arany et al Wound Rep Regen 2007, 15, 866). We observed a better organized healing response in laser irradiated oral tissues that correlated with an increased expression of TGF-beta1 immediately post laser irradiation. Our data suggested the source of latent TGF-beta1 might be from the degranulating platelets in the serum, an abundant source of in vivo latent TGF-beta, in the freshly wounded tissues. Further, we also demonstrated the ability of the low power near-infrared laser irradiation to activate the latent TGF-beta complexes in vitro at varying fluences from 10sec (0.1 J/cm2) to 600secs (6 J/cm2). Using serum we observed two isoforms, namely TGF-beta1 and TGF-beta3, were capable of being activated by laser irradiation using an isoform-specific ELISA and a reporter based (p3TP) assay system. We are presently pursuing the precise photomolecular mechanisms focusing on potential chromophores, wavelength and fluence parameters affecting the Latent TGF-beta activation process in serum. As ROS mediated TGF-beta activation has been previously demonstrated and we are also exploring the role of Laser generated-ROS in this activation process. In summary, we present evidence of a potential molecular mechanism for laser photobiomodulation in its ability to activate latent TGF-beta complexes.

Paper Details

Date Published: 18 February 2009
PDF: 8 pages
Proc. SPIE 7165, Mechanisms for Low-Light Therapy IV, 71650C (18 February 2009); doi: 10.1117/12.809839
Show Author Affiliations
Praveen R. Arany, Harvard Univ. (United States)
Massachusetts General Hospital (United States)
Aaron Chih-Hao Chen, Massachusetts General Hospital (United States)
Tristan Hunt, Harvard Univ. (United States)
David J. Mooney, Harvard Univ. (United States)
Michael Hamblin, Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 7165:
Mechanisms for Low-Light Therapy IV
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

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